Keywords
culture media
edible mushroom
legume-based media
low-cost
How to Cite
Abstract
Mushroom farming provides economic opportunities, but in the Philippines, the high cost of commercial culture media hinders its development. This study aimed to determine whether locally available beans could serve as a cheaper nutrient source for cultivating Agrocybe aegerita in Tabuk City. In this study, researchers measured the mycelial diameter of A. aegerita on five media: Water Agar (T−), Potato Dextrose Agar (T+), and black bean (T1), mung bean (T2), and white bean (T3) agar. A One-Way ANOVA followed by Tukey’s HSD test was applied at the 5% significance level. The species was identified on the basis of its appearance, and the media were prepared by boiling the beans to obtain a broth. The results indicated that PDA (T+) gave the greatest mycelial diameter (49.63 ± 13.28 mm), followed by black bean (31.87 ± 0.517 mm), mung bean (29.09 ± 0.522 mm), and white bean (27.19 ± 0.522 mm) agars. All the legume-based media outperformed the negative control. Higher humidity also slightly inhibited mycelial growth. Although PDA remained the most effective, agars made from black, mung, and white beans are viable alternatives. The study suggests that further research should be carried out to improve methods of media preparation and to promote affordable mushroom production in rural and low-resource areas.
References
Adolfsson, L., Nziengui, H., Abreu, I. N., Šimura, J., Beebo, A., Herdean, A., Aboalizadeh, J., Široká, J., Moritz, T., Novák, O., Ljung, K., Schoefs, B., & Spetea, C. (2017). Enhanced secondary and hormone metabolism in leaves of arbuscular mycorrhizal Medicago truncatula. Plant Physiology, 175(1), 392–411. https://doi.org/10.1104/pp.16.01509
Alam, N., Kim, J. H., Shim, M. J., Lee, U. Y., & Lee, T. S. (2010). Mycelial propagation and molecular phylogenetic relationships of commercially cultivated Agrocybe cylindracea based on ITS sequences and RAPD. Mycobiology, 38(2), 89–96. https://doi.org/10.4489/myco.2010.38.2.089
Al-Asmari, A. R. K., Siddiqui, Y. M., Athar, M. T., Al-Buraidi, A., Al-Eid, A. S., & Horaib, G. B. (2015). Antimicrobial activity of aqueous and organic extracts of a Saudi medicinal plant: Rumex nervosus. Journal of Pharmacy and Bioallied Sciences, 7(4), 300–303. https://doi.org/10.4103/0975-7406.168031
Ballesteros, J., Manaois, R., Morales, A., & Abilgos-Ramos, R. (2021). Towards consumer-oriented mushroom-based product development: An exploratory study in rice-based farming communities in Central Luzon, Philippines. Journal of Economics, Management and Agricultural Development, 7, 1–19. https://www.ukdr.uplb.edu.ph/jemad/vol7/iss1/1
Bellettini, M. B., Fiorda, F. A., Maieves, H. A., Teixeira, G. L., Ávila, S., Hornung, P. S., Júnior, A. M., & Ribani, R. H. (2019). Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26(4), 633–646. https://doi.org/10.1016/j.sjbs.2016.12.005
Bhatia, S., Sharma, K., Dahiya, R., & Bera, T. (2015). Modern applications of plant biotechnology in pharmaceutical sciences. Academic Press. https://doi.org/10.1016/C2014-0-02123-5
Chang, H. Y., Jeon, S. W., Cosadio, A., Icalina, C., Panganiban, R., Quirino, R., & Song, Y. (2014). Status and prospect of mushroom industry in the Philippines. JPAIR Multidisciplinary Research, 16(1), 1–16. https://doi.org/10.7719/jpair.v16i1.268
Condrillon, C., Masong, L., Sandoval, C. E., Siojo, C., & Bustamante, J. (2024). Vigna radiata (mung beans) as an alternative culture medium for Trypticase Soy Agar. Asian Journal Of Medical Technology, 4(1), 1-21. https://doi.org/10.32896/ajmedtech.v4n1.1-21
Dulay, R. M. R., & Damaso, E. J., Jr. (2020). The successful cultivation of Philippine wild mushroom Pycnoporus sanguineus (BIL7137) using rice straw- and sawdust-based substrate. Journal of Applied Biology and Biotechnology. https://doi.org/10.7324/JABB.2020.80509
Dulay, R. M. R., & Damaso, E. J., Jr. (2020). The successful cultivation of Philippine wild mushroom Pycnoporus sanguineus (BIL7137) using rice straw and sawdust-based substrate. Journal of Applied Biology & Biotechnology, 8(5), 72–77. https://doi.org/10.7324/JABB.2020.80509
Dulay, R. M. R., Cabrera, E. C., Kalaw, S. P., & Reyes, R. G. (2021b). Optimization of culture conditions for mycelial growth and fruiting body production of naturally occurring Philippine mushroom Lentinus swartzii Berk. Journal of Applied Biology and Biotechnology, 9(3), 17–25. https://doi.org/10.7324/JABB.2021.9303
Ene, I. V., Walker, L. A., Schiavone, M., Lee, K. K., Martin-Yken, H., Dague, E., & Brown, A. J. P. (2015). Cell wall remodeling enzymes modulate fungal cell wall elasticity and osmotic stress resistance. mBio, 6(4), e00986-15. https://doi.org/10.1128/mBio.00986-15
Falandysz, J., Fernandes, A. R., & Zhang, J. (2024). Critical review of rare earth elements (REE) in cultivated macrofungi. Food Control, 155, 110085. https://doi.org/10.1016/j.foodcont.2023.110085
Fujimoto, Y., Nagata, R., Fukasawa, H., Yano, K., Azuma, M., Iida, A., & Hashimoto, Y. (1998). Purification and cDNA cloning of cytokinin-specific binding protein from mung bean (Vigna radiata). European Journal of Biochemistry, 258(2), 794–802. https://doi.org/10.1046/j.1432-1327.1998.2580794.x
Gamit, T., Hajoori, M., & Maisuria, N. (2023). A review: Formulation of alternative culture media. International Journal of Life Science and Agriculture Research, 2(8), 206–212. https://doi.org/10.55677/ijlsar/V02I08Y2023-01
Gupta, D. K., Rühl, M., Mishra, B., Kleofas, V., Hofrichter, M., Herzog, R., & Thines, M. (2018). The genome sequence of the commercially cultivated mushroom Agrocybe aegerita reveals a conserved repertoire of fruiting-related genes and a versatile suite of biopolymer-degrading enzymes. BMC Genomics, 19, 1–13. https://doi.org/10.1186/s12864-017-4430-y
Hammerton, R. D., Nicander, E., & Tillberg, E. (1996). Identification of some major cytokinins in Phaseolus vulgaris and their distribution. Physiologia Plantarum, 96, 77–84. https://doi.org/10.1111/j.1399-3054.1996.tb00186.x
Hu, Y., Xue, F., Chen, Y., Qi, Y., Zhu, W., Wang, F., & Shen, J. (2023). Effects and mechanism of the mycelial culture temperature on the growth and development of Pleurotus ostreatus (Jacq.) P. Kumm. Horticulturae, 9(1), 95. https://doi.org/10.3390/horticulturae9010095
Islam, T., Zakaria, Z., Hamidin, N., & Ishak, M. A. B. M. (2016). Optimization of humidifying procedure in controlled environment for indoor cultivation of Pleurotus pulmonarius. African Journal of Biotechnology, 15(45), 2578–2586. https://doi.org/10.5897/AJB2016.15621
Jain, A., Yadav, S., Nigam, V. K., & Sharma, S. R. (2017). Fungal-mediated solid waste management: A review. In V. K. Gupta & M. Tuohy (Eds.), Mycoremediation and environmental sustainability (Vol. 1, pp. 153–170). Springer. https://doi.org/10.1007/978-3-319-68957-9_9
Kalaw, S. P., De Leon, A. M., Damaso, E. J., Jr., Ramos, J. C., del Rosario, M. A. G., Abon, M. D., & Reyes, R. G. (2021). Cultivation of different strains of Lentinus tigrinus from selected areas of Luzon Island, Philippines. Studies in Fungi, 6(1), 299–306. https://doi.org/10.5943/sif/6/1/20
Kebede, E. (2021). Contribution, utilization, and improvement of legumes-driven biological nitrogen fixation in agricultural systems. Frontiers in Sustainable Food Systems, 5, 767998. https://doi.org/10.3389/fsufs.2021.767998
Lyu, A., Yang, L., Wu, M., Zhang, J., & Li, G. (2020). High efficacy of the volatile organic compounds of Streptomyces yanglinensis 3-10 in suppression of Aspergillus contamination on peanut kernels. Frontiers in Microbiology, 11, 142. https://doi.org/10.3389/fmicb.2020.00142
Miles, P. G., & Chang, S. T. (2004). Mushrooms: Cultivation, nutritional value, medicinal effect, and environmental impact. CRC Press. https://doi.org/10.1201/9780203492086
Muthu, N., & Shanmugasundaram, K. (2015). Effect of five different culture media on mycelial growth of Agrocybe aegerita. International Journal of Pharmaceutical Sciences and Research, 6(12), 5193–5197. https://doi.org/10.13040/IJPSR.0975-8232.6(12).5193-97
Philippoussis, A., Zervakis, G., & Diamantopoulou, P. (2001). Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea, and Pleurotus spp. World Journal of Microbiology and Biotechnology, 17(2), 191–200. https://doi.org/10.1023/A:1016685530312
Phonemany, M., Niego, A. G. T., Hyde, K. D., Al-Otibi, F., & Thongklang, N. (2025). Screening and domestication of cultivable macrofungi: nutritional value, and industrial applications. Chiang Mai J Sci, 52, e2025054. https://doi.org/10.12982/CMJS.2025.054
Platon, N. A. D., Marquez, I. C., Mista, J. A. S., Javier, G. M., Custodio, K. R. D., & Amorado, C. E. S. (2025). Phaseolus vulgaris (Baguio bean) extract as an alternative nutrient source for the culture medium of selected fungal strains. Asia Pacific Journal of Allied Health Sciences, 8(1), 80–93. https://doi.org/10.70979/IRQP8865
Jintapattanakit, A., Teerapatsakul, C., & Thachepan, S. (2021). Putative hydrophobins of black poplar mushroom (Agrocybe cylindracea). Mycology, 12(1), 58-67. https://doi.org/10.1080/21501203.2020.1804474
Rukhiran, M., Sutanthavibul, C., Boonsong, S., & Netinant, P. (2023). IoT-based mushroom cultivation system with solar renewable energy integration: Assessing the sustainable impact of the yield and quality. Sustainability, 15(18), 13968. https://doi.org/10.3390/su151813968
Santos, F., Magalhães, D., Nascimento, J., & Ramos, G. (2021). Use of products of vegetable origin and waste from hortofruticulture for alternative culture media. Food Science and Technology, 42, e00621. https://doi.org/10.1590/fst.00621
Semba, R. D., Ramsing, R., Rahman, N., Kraemer, K., & Bloem, M. W. (2021). Legumes as a sustainable source of protein in human diets. Global Food Security, 28, 100520. https://doi.org/10.1016/j.gfs.2021.100520
Syamsia, S., Idhan, A., Latifah, H., Noerfityani, N., & Akbar, A. (2021, November). Alternative medium for the growth of endophytic fungi. In IOP Conference Series: Earth and Environmental Science (Vol. 886, No. 1, p. 012045). IOP Publishing. https://doi.org/10.1088/1755-1315/886/1/012045
Talley, S. M., Coley, P. D., & Kursar, T. A. (2002). The effects of weather on fungal abundance and richness among 25 communities in the Intermountain West. BMC Ecology, 2, Article 7. https://doi.org/10.1186/1472-6785-2-7
Thakur, M. P. (2020). Advances in mushroom production: Key to food, nutritional and employment security: A review. Indian Phytopathology, 73, 377–395. https://doi.org/10.1007/s42360-020-00244-9
Voutetaki, M. E., & Mpalaskas, A. C. (2024). Natural fiber-reinforced mycelium composite for innovative and sustainable construction materials. Fibers, 12(7), 57. https://doi.org/10.3390/fib12070057
Wani, I. A., Sogi, D. S., Hamdani, A. M., Gani, A., Bhat, N. A., & Shah, A. (2016). Isolation, composition, and physicochemical properties of starch from legumes: A review. Starch - Stärke, 68(9–10), 834–845. https://doi.org/10.1002/star.201600007
Widyanur, H., Sasongkowati, R., & Suliati, S. (2023). Utilization of green beans (Vima 1 and local) as an alternative media substitute for Sabouraud dextrose agar (SDA) in the growth of Trichophyton rubrum. Journal of Health Science, 16(1), 8–11. https://doi.org/10.33086/jhs.v16i01.3181
Zak, J. C., & Wildman, H. G. (2004). Fungi in stressful environments. In G. M. Mueller, G. F. Bills, & M. S. Foster (Eds.), Biodiversity of fungi (pp. 303–315). Elsevier Academic Press. https://doi.org/10.1016/B978-012509551-8/50017-9
Zhan, Z., Meiling, X., Li, Y., & Dong, M. (2021). The relationship between fungal growth rate and temperature and humidity. International Journal of Engineering and Management Research, 11(3), 78-83. https://doi.org/10.31033/ijemr.11.3.13
Zhang, J., & Elser, J. J. (2017). Carbon:nitrogen:phosphorus stoichiometry in fungi: A meta-analysis. Frontiers in Microbiology, 8, 1281. https://doi.org/10.3389/fmicb.2017.01281
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