Keywords
boar semen
coconut water
Duroc
honey
large white
mCASA
organic semen extender
sperm motility
time-series design
How to Cite
Abstract
This study focused on evaluating the motility rate of semen from Large White and Duroc breeds using organic semen extenders—specifically coconut water and honey—compared to a commercial extender used as the control. It explored the composition of these organic extenders, the morphology of boar semen based on normal fraction, and motility rate, which was assessed using a mobile computer-assisted sperm analyzer (mCASA). This study employed a Multiple Time Series Design (MTSD), wherein evaluations were both performed before processing, with the sperm temperature at 35 °C, and after processing. The samples were monitored at 18 °C from 8 a.m. to 8 p.m. at 2-hour intervals. Monitoring occurred after 2, 4, 6, 8, 10, and 12 hours across all treatments, including the control. Results showed that honey-based Treatment 4 best preserved motility in Large White boar semen for up to 12 hours, outperforming all other treatments, including the commercial extender. In contrast, coconut water-based Treatment 1 maintained high motility in Duroc semen for up to 48 hours, exceeding both commercial and organic alternatives. These findings suggest breed-specific responses to extenders and highlight the potential of Treatments 4 and 1 as effective, organic option. Future studies should consider genotype-specific traits to optimize semen preservation.
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